Video Channel

Filmed live at the 2026 Abundance360 Summit, Sinclair covers how AI is compressing drug-discovery timelines from decades to months, the real risks and benefits of GLP-1 agonists for longevity, and where the smartest longevity capital is flowing. He also shares his updated biological-age reversal data and near-term timeline for a clinically approved age-reversal intervention.

Johnson presents his biological age test results across multiple epigenetic clocks, showing consistent reversal of biological age over 2 years of the Blueprint protocol. He discusses the Rejuvenation Olympics leaderboard, the metrics he tracks, and why he believes the protocol is replicable at lower cost.

A wide-ranging conversation covering Johnson's 'Don't Die' philosophy, the Blueprint protocol's evolution, and his views on the future of human longevity. He discusses the tension between extreme self-optimisation and quality of life, and why he believes algorithmic decision-making about health will become mainstream.

Sinclair presents the latest results from his lab on partial reprogramming using OSK (Oct4, Sox2, Klf4) Yamanaka factors, showing reversal of epigenetic age in multiple tissues including the retina, brain, and muscle. He discusses the timeline for human applications and the safety profile of partial (vs full) reprogramming.

Johnson outlines his Blueprint protocol — a rigorous self-experimentation programme designed to minimise biological age. He covers his daily measurement stack, dietary approach (vegan, calorie-controlled), sleep optimisation, and the biomarker data showing reversal of several biological age markers. Presented as a practitioner account, not peer-reviewed research.

Johnson discusses the Blueprint protocol in full: his $2M/year self-experiment, the 100+ monthly biomarkers, the team of 30 physicians, and his philosophical framework of 'don't die.' He covers his diet (17:7 fasting, plant-forward, no alcohol), sleep protocol (strict 8-hour window), and why he believes the human body can be optimised like software.

Patrick reviews the Finnish cohort data showing 40% reduction in cardiovascular mortality with 4–7 sauna sessions per week, the heat shock protein (HSP) response, and why sauna mimics many of the benefits of aerobic exercise. She covers optimal temperature (80–100°C), duration (15–20 min), and the evidence for contrast therapy.

Patrick covers her research on sulforaphane — the NRF2-activating compound in broccoli sprouts — and its effects on cancer prevention, neuroinflammation, and aging. She explains how to maximise sulforaphane content from broccoli sprouts (myrosinase activation) and why the supplement form varies widely in quality.

Dr Layne Norton, PhD biochemistry, reviews the evidence on protein requirements for muscle maintenance, fat loss, and longevity. He addresses common myths about protein and kidneys, and explains why the RDA of 0.8g/kg is a minimum for survival, not an optimum for health.

Attia presents the core thesis of Outlive: that Medicine 3.0 requires proactive, personalised intervention decades before disease manifests. He argues that exercise — specifically Zone 2 cardio and strength training — is the single most powerful longevity intervention, and that ApoB and Lp(a) testing should be universal.

Dr Nir Barzilai, director of the Institute for Aging Research at Einstein College of Medicine, explains the TAME (Targeting Aging with Metformin) trial — the first FDA-approved clinical trial to test a drug that targets aging itself rather than a specific disease.

Sinclair walks through his morning routine: skipping breakfast, morning NMN + resveratrol + TMG, cold exposure, and why he avoids eating early in the day. He explains the science of mTOR suppression through fasting and why he believes skipping breakfast is one of the highest-leverage longevity habits.

Dr Gabrielle Lyon, founder of muscle-centric medicine, argues that skeletal muscle is the primary organ of longevity and metabolic health. She covers the evidence for protein intake, resistance training, and why the focus on fat loss is misplaced — the real target is muscle gain.

Huberman presents his evidence-based exercise protocol for longevity: 3 days of resistance training, 2 days of Zone 2 cardio, 1 day of VO₂max training, and 1 rest day. He covers the neuroscience of exercise — BDNF, IGF-1, and the anti-inflammatory effects of regular physical activity.

Dr Morgan Levine, Yale epigenetics researcher and author of *True Age*, explains how biological age clocks work, what they measure, and how lifestyle interventions can slow or reverse biological aging. She covers PhenoAge, GrimAge, and the latest multi-omic aging clocks.

Patrick makes the case that vitamin D deficiency (affecting ~40% of the US population) is a significant, modifiable longevity risk factor. She covers the 2000+ genes regulated by vitamin D, the VITAL trial data, and why the RDA of 600 IU is almost certainly too low for optimal health.

Kaeberlein, one of the world's leading aging researchers, covers the ITP rapamycin data, the Dog Aging Project, and the state of human evidence for mTOR inhibition. He argues that rapamycin is the most promising longevity drug currently available and explains why the risk-benefit calculation favours use in healthy adults.

Galpin explains the mechanisms of muscle hypertrophy, strength adaptation, and recovery. He covers how muscle mass is one of the strongest predictors of longevity and metabolic health, and provides evidence-based protocols for building and maintaining muscle across the lifespan.

Dr Andy Galpin, exercise physiologist at Cal State Fullerton, delivers a masterclass on building endurance. He covers the physiology of VO2 max, zone 2 training, and why cardiovascular fitness is the single strongest predictor of all-cause mortality — stronger than any supplement.

Patrick covers the VITAL trial data on omega-3 and cancer/cardiovascular outcomes, the REDUCE-IT trial showing 25% reduction in cardiovascular events with 4g EPA, and the emerging evidence for DHA in brain health and dementia prevention. She explains why the omega-3 index is a critical biomarker.

Huberman's comprehensive sleep toolkit covers the neuroscience of sleep, the role of adenosine and cortisol in sleep-wake regulation, and a practical 12-step protocol for optimising sleep quality. He explains why morning sunlight exposure is the single most powerful sleep intervention and how to use light, temperature, and supplements strategically.

A wide-ranging conversation covering Sinclair's career, the science behind Lifespan, and his predictions for longevity medicine. He discusses the TAME trial (metformin for aging), partial reprogramming, and why he believes the first person to live to 150 is already alive.

Dr George Church, professor of genetics at Harvard Medical School, discusses the frontier of genome engineering — from CRISPR-based gene therapies targeting aging hallmarks to recoding the human genome for viral resistance. He covers his lab's work on multiplex gene editing, xenotransplantation, and a combination gene therapy that reversed multiple age-related diseases in mice.

Longo presents the science behind the Fasting Mimicking Diet (FMD) — a 5-day, low-calorie protocol that triggers autophagy and stem cell regeneration while allowing continued food intake. He covers the human trial data showing reductions in biological age markers, IGF-1, and visceral fat after 3 monthly FMD cycles.

Walker addresses the 'J-curve' myth of alcohol and health, explaining why even moderate alcohol consumption impairs sleep quality, suppresses REM sleep, and accelerates biological aging. He covers the Mendelian randomisation data showing no safe level of alcohol for health.

Attia interviews Matt Kaeberlein on rapamycin's mechanism of action, the ITP (Interventions Testing Program) data showing lifespan extension in mice, and the current state of human evidence. They discuss dosing protocols, side effects, and why Attia uses rapamycin weekly in his own practice.

Huberman covers the neuroscience of cold exposure: the norepinephrine surge (up to 300%), dopamine elevation (lasting hours), and metabolic benefits of cold-induced brown adipose tissue activation. He provides a practical protocol (1–3 min at uncomfortably cold temperature, 2–3× per week) and explains why cold after strength training may blunt hypertrophy.

Attia explains the physiology of Zone 2 cardio — the intensity at which fat oxidation is maximised and mitochondrial biogenesis is stimulated. He covers how to identify Zone 2 (lactate threshold 1, conversational pace), why 3–4 hours per week is the minimum effective dose, and how to measure progress via lactate testing.

Sinclair presents his Information Theory of Aging — the idea that aging is fundamentally a loss of epigenetic information, not a consequence of DNA damage alone. He covers his personal supplement stack (NMN, resveratrol, metformin), the OSK reprogramming experiments in mice, and why he believes biological age reversal is achievable in humans within a decade.

Attia and Matthew Walker cover the full science of sleep: why 7–9 hours is non-negotiable, what happens during deep sleep (glymphatic clearance of amyloid-beta), and how sleep deprivation accelerates every hallmark of aging. Practical protocols for improving sleep quality are discussed in detail.

Huberman covers the neuroscience of stress — the HPA axis, cortisol, and adrenaline — and provides a toolkit of evidence-based tools for real-time stress reduction (physiological sigh), medium-term stress inoculation (deliberate cold/exercise), and long-term stress management (NSDR, social connection). He explains why some stress is beneficial and how to calibrate the dose.

In this follow-up conversation, Panda covers the human clinical trial data on TRE, the interaction between sleep and eating windows, and why the timing of the first and last meal matters as much as what is eaten. He discusses the TREAT trial and his myCircadianClock app used to study TRE in real-world populations.

Walker, author of Why We Sleep, covers the full biology of sleep: glymphatic clearance, memory consolidation, immune function, and the catastrophic health consequences of chronic sleep deprivation. He provides practical tools for improving sleep and explains why sleep is the single most important health behaviour.

Sapolsky, author of Why Zebras Don't Get Ulcers, explains why chronic psychological stress is uniquely damaging to human health — unlike the acute stress experienced by other animals. He covers the HPA axis, glucocorticoid toxicity, telomere shortening under stress, and why social hierarchy and perceived control are the strongest predictors of stress-related disease.

Dr Mark Mattson, former chief of the Laboratory of Neurosciences at the National Institute on Aging, explains the neuroscience of intermittent fasting — how metabolic switching from glucose to ketones activates BDNF, autophagy, and stress-resistance pathways in the brain. He covers the evidence for fasting in Alzheimer's prevention and cognitive performance.

Dr Steve Horvath, creator of the Horvath epigenetic clock, explains how DNA methylation patterns can predict biological age with remarkable accuracy across all tissues, and what this means for longevity research. He covers the development of the pan-tissue clock, the GrimAge clock, and the emerging evidence that biological age can be reversed by lifestyle and pharmacological interventions.

Dr Guido Kroemer, one of the world's leading cell biologists and a pioneer of autophagy research, explains the molecular mechanisms by which caloric restriction, fasting, and rapamycin extend lifespan. He covers the role of autophagy in clearing damaged organelles, the AMPK/mTOR axis, and why spermidine — a natural polyamine — is one of the most promising autophagy-inducing longevity compounds.

Dr Eric Verdin, president of the Buck Institute for Research on Aging, explains how a cyclic ketogenic diet extended lifespan and improved memory in aged mice, and how beta-hydroxybutyrate (BHB) acts as a signalling molecule that inhibits HDAC enzymes, upregulates FOXO3, and reduces inflammation. He also covers the role of NAD+ decline in aging and the evidence for NR supplementation.

Dr Satchin Panda, professor at the Salk Institute, explains the science of time-restricted eating (TRE) and why aligning food intake with the body's circadian clock is a powerful longevity intervention. He covers his landmark mouse studies showing that mice eating within an 8–10 hour window were protected from obesity, diabetes, and liver disease even on a high-fat diet.

Dr Elissa Epel, professor at UCSF and co-author of The Telomere Effect, explains how chronic psychological stress accelerates cellular aging by shortening telomeres and reducing telomerase activity. She covers the landmark caregiver study showing 10 years of additional biological aging from chronic stress, and practical interventions — mindfulness, exercise, and omega-3 — that protect telomere length.

Dr Valter Longo's landmark FoundMyFitness conversation covers the Fasting Mimicking Diet (FMD) — a 5-day, low-calorie protocol that triggers fasting biology including stem cell regeneration and immune system reset. He presents mouse and early human data showing the FMD reduces IGF-1, visceral fat, and biological age markers, and discusses its potential in cancer and autoimmune disease treatment.

Dr Judith Campisi, professor at the Buck Institute for Research on Aging, explains the dual role of cellular senescence — protective against cancer in the short term, but driving inflammation and tissue dysfunction in the long term. She covers the SASP (senescence-associated secretory phenotype), the evidence for senolytics, and why clearing senescent cells extends healthspan in mice.

Dr Aubrey de Grey, biomedical gerontologist and founder of the SENS Research Foundation, outlines his framework for defeating aging: identifying and repairing the seven categories of cellular and molecular damage that accumulate over a lifetime. He argues that aging is an engineering problem, not a biological inevitability, and that CRISPR is the most important recent advance for SENS therapies.